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lentiviral vectors for tetracycline-inducible short hairpin rna expression  (BioResource International Inc)
90
BioResource International Inc lentiviral vectors for tetracycline-inducible short hairpin rna expression
Lentiviral Vectors For Tetracycline Inducible Short Hairpin Rna Expression, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
lentiviral vectors for tetracycline-inducible short hairpin rna expression - by Bioz Stars, 2026-10
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lentiviral vectors for tetracycline (tet)-inducible short hairpin rna (shrna) expression  (BioResource International Inc)
90
BioResource International Inc lentiviral vectors for tetracycline (tet)-inducible short hairpin rna (shrna) expression
LSD2 is essential for BAT differentiation in HB2 cells. A, B) Dox-inducible KD of LSD2. HB2 cells were transduced with tet-inducible shLSD2 (LSD2-KD#1 and #2) or control (shCtr) lentiviral vectors followed by blasticidin selection. Cells were cultured with or without Dox for 48 h. For relative mRNA levels of LSD2 (A), real-time quantitative PCR values were normalized to values for the 36B4 gene and are shown as fold differences against Dox (−). For levels of LSD2 protein (B), β-tubulin was used as a loading control. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01. C) Oil Red O staining of <t>shRNA-expressing</t> HB2 cells at d 7. Culture details are described in Supplemental Fig. S1A. Scale bars, 100 µm. D) Expression of adipogenesis and BAT-selective genes in shLSD2_#1 cells at indicated days. Quantitative RT-PCR values were normalized to those for the 36B4 gene and are shown as fold differences against Dox (−) at d 0. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01 vs. Dox (−) at each time point.
Lentiviral Vectors For Tetracycline (Tet) Inducible Short Hairpin Rna (Shrna) Expression, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inducible+expression+lentiviral+vector/lentiviral+vectors+for+tetracycline++tet++inducible+short+hairpin+rna++shrna++expression/pmc06436657-94-11-23
Average 90 stars, based on 1 article reviews
lentiviral vectors for tetracycline (tet)-inducible short hairpin rna (shrna) expression - by Bioz Stars, 2026-10
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pmin-ducer, a custom synthesized lentiviral vector that provides doxycycline-inducible control of transgene expression  (GenScript corporation)
90
GenScript corporation pmin-ducer, a custom synthesized lentiviral vector that provides doxycycline-inducible control of transgene expression
LSD2 is essential for BAT differentiation in HB2 cells. A, B) Dox-inducible KD of LSD2. HB2 cells were transduced with tet-inducible shLSD2 (LSD2-KD#1 and #2) or control (shCtr) lentiviral vectors followed by blasticidin selection. Cells were cultured with or without Dox for 48 h. For relative mRNA levels of LSD2 (A), real-time quantitative PCR values were normalized to values for the 36B4 gene and are shown as fold differences against Dox (−). For levels of LSD2 protein (B), β-tubulin was used as a loading control. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01. C) Oil Red O staining of <t>shRNA-expressing</t> HB2 cells at d 7. Culture details are described in Supplemental Fig. S1A. Scale bars, 100 µm. D) Expression of adipogenesis and BAT-selective genes in shLSD2_#1 cells at indicated days. Quantitative RT-PCR values were normalized to those for the 36B4 gene and are shown as fold differences against Dox (−) at d 0. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01 vs. Dox (−) at each time point.
Pmin Ducer, A Custom Synthesized Lentiviral Vector That Provides Doxycycline Inducible Control Of Transgene Expression, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inducible+expression+lentiviral+vector/pmin+ducer++a+custom+synthesized+lentiviral+vector+that+provides+doxycycline+inducible+control+of+transgene+expression/pm34492225-234-15-28
Average 90 stars, based on 1 article reviews
pmin-ducer, a custom synthesized lentiviral vector that provides doxycycline-inducible control of transgene expression - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

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LSD2 is essential for BAT differentiation in HB2 cells. A, B) Dox-inducible KD of LSD2. HB2 cells were transduced with tet-inducible shLSD2 (LSD2-KD#1 and #2) or control (shCtr) lentiviral vectors followed by blasticidin selection. Cells were cultured with or without Dox for 48 h. For relative mRNA levels of LSD2 (A), real-time quantitative PCR values were normalized to values for the 36B4 gene and are shown as fold differences against Dox (−). For levels of LSD2 protein (B), β-tubulin was used as a loading control. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01. C) Oil Red O staining of shRNA-expressing HB2 cells at d 7. Culture details are described in Supplemental Fig. S1A. Scale bars, 100 µm. D) Expression of adipogenesis and BAT-selective genes in shLSD2_#1 cells at indicated days. Quantitative RT-PCR values were normalized to those for the 36B4 gene and are shown as fold differences against Dox (−) at d 0. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01 vs. Dox (−) at each time point.

Journal: The FASEB Journal

Article Title: Lysine-specific demethylase-2 is distinctively involved in brown and beige adipogenic differentiation

doi: 10.1096/fj.201801422RR

Figure Lengend Snippet: LSD2 is essential for BAT differentiation in HB2 cells. A, B) Dox-inducible KD of LSD2. HB2 cells were transduced with tet-inducible shLSD2 (LSD2-KD#1 and #2) or control (shCtr) lentiviral vectors followed by blasticidin selection. Cells were cultured with or without Dox for 48 h. For relative mRNA levels of LSD2 (A), real-time quantitative PCR values were normalized to values for the 36B4 gene and are shown as fold differences against Dox (−). For levels of LSD2 protein (B), β-tubulin was used as a loading control. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01. C) Oil Red O staining of shRNA-expressing HB2 cells at d 7. Culture details are described in Supplemental Fig. S1A. Scale bars, 100 µm. D) Expression of adipogenesis and BAT-selective genes in shLSD2_#1 cells at indicated days. Quantitative RT-PCR values were normalized to those for the 36B4 gene and are shown as fold differences against Dox (−) at d 0. Values are mean ± sd (n = 3). *P < 0.05, †P < 0.01 vs. Dox (−) at each time point.

Article Snippet: Lentiviral expression of short hairpin RNA Lentiviral vectors for tetracycline (tet)-inducible short hairpin RNA (shRNA) expression were constructed using plasmids obtained from Riken BioResource Research Center (Saitama, Japan) according to the guidelines ( http://cfm.brc.riken.jp/Lentiviral_Vectors_J ).

Techniques: Transduction, Control, Selection, Cell Culture, Real-time Polymerase Chain Reaction, Staining, shRNA, Expressing, Quantitative RT-PCR